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Biology and morphometrics of the immature stages of Epinotia aporema on artificial diet PAB
Arneodo,Joel; Quintana,Graciela; Sciocco-Cap,Alicia.
The objective of this work was to establish a life table for the immature stages of Epinotia aporema, as part of a wider investigation on its biological control. Insects were reared on an artificial diet at 25±1ºC and a 16:8 (light:dark) hour photoperiod. For the identification of larval instars for the study of pathogen-insect interactions under laboratory conditions, head capsule widths (HCWs) were also determined. The egg incubation period was 4.13±0.30 days, larval stage took 11.64±0.49 days, and the development time of the pupal phase was sex-dependent with 8.51±0.69 days for the females and 9.41±0.65 days for the males. Five larval instars were identified.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Bean shoot borer; Cephalic capsule; Laboratory rearing; Larvae.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000200014
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Natural infection of Viola cornuta (Violaceae) with Cucumber mosaic virus, subgroup I Biocell
Arneodo,Joel; de Breuil,Soledad; Lenardon,Sergio; Conci,Luis.
Plants of Viola cornuta displaying typical virus symptoms were observed during spring 2003 in a plant nursery in Córdoba, central Argentina. Electron microscopic examinations of symptomatic leaf samples revealed the presence of isometric virus-like particles about 30 nm in diameter. Subsequent serological analysis allowed the identification of the pathogen as a subgroup I strain of Cucumber mosaic virus (CMV). These results were confirmed by antigen capture - reverse transcription - polymerase chain reaction with specific CMV primers, and digestion with a restriction enzyme. This is the first report of CMV infecting V. cornuta in Argentina.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Viola cornuta; Cucumber mosaic virus; Electron microscopy; Serology; AC-RT-PCR.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200008
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Transmisión del Mal de Río Cuarto virus por ninfas de primer y tercer estadio de Delphacodes kuscheli PAB
Arneodo,Joel; Guzmán,Fabiana; Ojeda,Silvia; Ramos,María Laura; Laguna,Irma; Conci,Luis; Truol,Graciela.
Se comparó la capacidad de ninfas de primer y tercer estadío de Delphacodes kuscheli Fennah (Hemiptera: Delphacidae) para adquirir y posteriormente transmitir el Mal de Río Cuarto virus (MRCV), bajo condiciones controladas. Ninfas I y III avirulíferas se alimentaron separadamente de plantas de trigo infectadas durante 48 horas, para luego ser colocadas en subgrupos de tres individuos sobre plantas de trigo sanas. Se realizaron transmisiones seriales utilizando períodos de inoculación de 24 horas. Ambos estadií os lograron adquirir y transmitir el MRCV, pero se evidenció una mayor cantidad de subgrupos infectivos cuando la adquisición se efectuó como ninfas I, así como una disminución significativa en la duración del período de latencia del virus respecto...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Delphacidae; Reoviridae; Transmisión experimental; Período de latencia.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000200014
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